National infrastructure FoodOmicsGR_RI, a multidisciplinary consortium, is mainly focused on the comprehensivecharacterization of different food matrices, method development and implementation of dietary studies, supportingthe AgroFood sectors. The RI combines strong expertise in basic science, advanced analytical techniques and bioinformatics with applied sciences, such as plant growth, oenology, animal husbandry, apiculture, food/nutrition science and dairy products. One of the main objectives is the holistic analysis of food content, the promotion of Greek products’ value and the study of possible effect of nutritional intervention on the metabolic/lipidomic-proteomic-genomic profile of biological samples of humans or/and animal cohorts. In the years of FoodOmicsGR operation, both targeted and untargeted omics protocols, using advanced analytical platforms, such as LC-MS/MS, GC-MS, LC/GC-QToF-MS, LC-TIMS-QToFMS, MALDI-ToFMS have been recruited for the discovery of potential (bio)markers in relation tofood analysis, authenticity, geographical and botanological origin, health claims, quality and safety and many more other examples, in a plethora of matrices (honey, dairy products, meat, olive oil, etc). Extensive databases were also constructed including targeted endogenous food metabolites.