This study compared physical activity (PA) - that is, daily employment, transfer to and from daily employment, leisure activities, and regular sports activities - before (PRE) and during (POST) ongoing COVID-19 epidemic in the exclusion environment in Greece. A Greek version of the Active-Q online questionnaire was used to access the PA. The questionnaire was completed twice (PRE and POST) by 8495 participants (age = 37.2 ± 0.2 years men = 38.3% and women = 61.7%). The relative frequency of total sports activities and overall participation in competitive sports decreased significantly (8.6% and 84.7% respectively). Activities decreased significantly (p <0.05). Total PA decreased in all sexes, age, body mass index (BMI) and PA level subgroups in the POST state and an interaction was observed between men and the High PA subgroups. The change in total PA (from PRE to POST conditions) was -16.3%, while in daily occupancy in transport and sports activities, it was -52.9%, -41.1% and -23.9% respectively . Thus, the exclusion period is largely associated with a negative change in overall PA. During the lockdown, inactivity increased dramatically, with men and the high PA population being significantly more affected. Thus, the lockdown period is highly associated with a negative change in overall PA.