Secondary plant metabolites, such as flavonoids, have been associated with several health benefits for the human organism^1,2,3. For this reason there is an ever-growing interest in the food industry for natural products that are rich in bioactive constituents. Roses (Rosa L.), the most widely cultivated flower in the world, have always been appreciated for their unique organoleptic properties. However, there is limited information available concerning their phytochemical profile as well as their antioxidant capacity, and their potential use as an alternative source of natural ingredients in the food and cosmetics industry. In this study, a rapid HPLC-UV protocol was developed to determine the concentrations of quercitrin in six different genotypes of red roses. Furthermore, DPPH assay was applied to measure total antioxidant capacity. ANOVA statistical analysis was applied to the results, which showed no significant statistical differences among the genotypes in terms of total antioxidant capacity. The ANOVA test revealed, however, significant statistical differences in the concentrations of quercitrin among the genotypes, implying that the concentrations of individual phenolic compounds vary among the genotypes.